The UV-Vis spectrum is due to the electronic transitions of the molecule which is characteristic of a compound. Qualitative and Quantitative estimations of a compound can be attained by this non-destructive technique. The absorbance (A) of a solution at a particular wavelength is determined by the Lambert Beer’s Law: A = εcl, where A is the absorbance of the compound, ε is the molar extinction coefficient of the compound at a particular wavelength, c is the concentration of the compound and l is the thickness of the cell. The quantitative measurements are based on this principle.

Application:

Qualitative and Quantitative determination of a compound can be achieved. Additionally the structure of a molecule, plausible reaction pathway of interaction between the host molecule and the incoming analyte, kinetics of a reaction, generation of colour centres can be accessed by this instrument.